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anti cd16 cd32  (Bio X Cell)


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    Structured Review

    Bio X Cell anti cd16 cd32
    Anti Cd16 Cd32, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd16+cd32+antibody/InVivoMAb+anti-mouse+CD16+CD32/pm41912083-75-20-25
    Average 96 stars, based on 357 article reviews
    anti cd16 cd32 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: ASFV Activates STAT3 to Induce Proviral M2 Macrophage Polarization
    Article Snippet: African swine fever (ASF) is an acute and highly infectious disease caused by African swine fever virus (ASFV) that has dealt a massive blow to the development of the pig industry in China.. Macrophages, the primary target cells of ASFV, exhibit high plasticity, however, their phenotypic changes during infection remain poorly understood.. In this study, we observed a significant increase in M2 monocytes within the peripheral blood cells of ASFV-infected pigs.

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism
    Article Snippet: .. After incubation with Zombie-Aqua (BioLegend) for dead cell staining and anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, the cells were incubated with 20 μL recipient plasma for 30 min. After washing, the splenocytes were incubated with Alexa Fluor 488-conjugated anti-mouse IgG (A-21202, Thermo Fisher, 1:150) or Alexa Fluor 488-conjugated anti-mouse IgM (ab150121, Abcam, 1:150) immunofluorescent antibody for another 30 min. Data were acquired using a flow cytometer (CytoFlex; Beckman Coulter, Inc.). ..

    Single Cell:

    Article Title: ASFV Activates STAT3 to Induce Proviral M2 Macrophage Polarization
    Article Snippet: African swine fever (ASF) is an acute and highly infectious disease caused by African swine fever virus (ASFV) that has dealt a massive blow to the development of the pig industry in China.. Macrophages, the primary target cells of ASFV, exhibit high plasticity, however, their phenotypic changes during infection remain poorly understood.. In this study, we observed a significant increase in M2 monocytes within the peripheral blood cells of ASFV-infected pigs.

    Article Title: Myeloid-Derived Suppressor Cells Induce Exhaustion-Like CD8 + T Cells during JEV Infection
    Article Snippet: .. Single-cell suspensions from the mouse spleen, lymph node, blood, and cultured cells were preincubated for 10 min at room temperature with 0.3 μg of anti-CD16/CD32 antibody (BioXcell). ..

    Staining:

    Article Title: The Glucose Transporter GLUT3 Controls Regulatory T Cell Function
    Article Snippet: .. Briefly, cells were stained with Fixable Viability Dye eFluor 780 (eBioscience) for 10 min in PBS at RT together with an anti-CD16/CD32 antibody (clone 2.4G2; Bio X Cell) to prevent non-specific binding. ..

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism
    Article Snippet: .. Cells were stained with Zombie-aqua (BioLegend) to discriminate dead cells, incubated with anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, and labeled using fluorescence-conjugated anti-mouse antibodies: CD3ε -PE/Dazzle 594 (clone 145-2C11, dilution 1:200, Cat 100347), CD4-Pacific blue (clone RM4-4, dilution 1:200, Cat 116008), B220-AlexaFluor700 (clone RA3-6B2, dilution 1:200, Cat 103232), CD138-PE (clone 281-2, dilution 1:200 Cat 142504), and CD8-APC/FireTM 750 (clone 53-6.7, dilution 1:200 Cat 100766). .. Data were acquired using a flow cytometer (LSR Fortessa; BD Biosciences, San Jose, CA, USA).

    Article Title: Tissue-resident neutrophils serve homeostatic and immunological functions in embryos
    Article Snippet: .. Prior to antibody staining, cells were stained with Fixable Viability Dye eFluor® 780 or 506 (65-0865-14 or 65-0866-14, respectively, Thermo Fisher) and Fc-receptor binding was blocked with anti-CD16/CD32 antibody (clone 2.4G2, BE0307, Bio X Cell). .. The cells were then incubated with fluorophore-conjugated primary antibodies diluted in flow cytometry buffer (PBS with 2% (v/v) fetal calf serum (FCS, S181B-500, Biowest) and 0.05% (v/v) sodium azide (S2002, Sigma-Aldrich)) at 4 °C for 30 min. Alternatively, unconjugated primary antibodies followed by appropriate fluorophore-conjugated secondary antibodies were used.

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism
    Article Snippet: .. After incubation with Zombie-Aqua (BioLegend) for dead cell staining and anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, the cells were incubated with 20 μL recipient plasma for 30 min. After washing, the splenocytes were incubated with Alexa Fluor 488-conjugated anti-mouse IgG (A-21202, Thermo Fisher, 1:150) or Alexa Fluor 488-conjugated anti-mouse IgM (ab150121, Abcam, 1:150) immunofluorescent antibody for another 30 min. Data were acquired using a flow cytometer (CytoFlex; Beckman Coulter, Inc.). ..

    Article Title: PD-L1- and IL-4-expressing basophils promote pathogenic accumulation of T follicular helper cells in lupus
    Article Snippet: For mouse samples, cells washed in PBS were stained with GHOST 510 viability dye (TONBO) following the manufacturer’s instructions. .. Non-specific antibody binding sites were saturated with 10 μg/mL of anti-CD16/CD32 antibody clone 2.4G2 (BioXCell), and 100 μg/mL of polyclonal rat, mouse, and Armenian Hamster IgG (Innovative Research Inc.) in FACS buffer and stained with the antibodies described in Supplementary Table for 30 min in the dark at 4 °C. ..

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism.
    Article Snippet: .. Cells were stained with Zombie-aqua (BioLegend) to discriminate dead cells, incubated with anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, and labeled using fluorescence-conjugated anti-mouse antibodies: CD3ε -PE/Dazzle 594 (clone 145-2C11, dilution 1:200, Cat 100347), CD4-Pacific blue (clone RM4-4, dilution 1:200, Cat 116008), B220AlexaFluor700 (clone RA3-6B2, dilution 1:200, Cat 103232), CD138-PE (clone 281-2, dilution 1:200 Cat 142504), and CD8-APC/FireTM 750 (clone 53-6.7, dilution 1:200 Cat 100766). .. Data were acquired using a flow cytometer (LSR Fortessa; BD Biosciences, San Jose, CA, USA).

    Binding Assay:

    Article Title: The Glucose Transporter GLUT3 Controls Regulatory T Cell Function
    Article Snippet: .. Briefly, cells were stained with Fixable Viability Dye eFluor 780 (eBioscience) for 10 min in PBS at RT together with an anti-CD16/CD32 antibody (clone 2.4G2; Bio X Cell) to prevent non-specific binding. ..

    Article Title: Tissue-resident neutrophils serve homeostatic and immunological functions in embryos
    Article Snippet: .. Prior to antibody staining, cells were stained with Fixable Viability Dye eFluor® 780 or 506 (65-0865-14 or 65-0866-14, respectively, Thermo Fisher) and Fc-receptor binding was blocked with anti-CD16/CD32 antibody (clone 2.4G2, BE0307, Bio X Cell). .. The cells were then incubated with fluorophore-conjugated primary antibodies diluted in flow cytometry buffer (PBS with 2% (v/v) fetal calf serum (FCS, S181B-500, Biowest) and 0.05% (v/v) sodium azide (S2002, Sigma-Aldrich)) at 4 °C for 30 min. Alternatively, unconjugated primary antibodies followed by appropriate fluorophore-conjugated secondary antibodies were used.

    Article Title: PD-L1- and IL-4-expressing basophils promote pathogenic accumulation of T follicular helper cells in lupus
    Article Snippet: For mouse samples, cells washed in PBS were stained with GHOST 510 viability dye (TONBO) following the manufacturer’s instructions. .. Non-specific antibody binding sites were saturated with 10 μg/mL of anti-CD16/CD32 antibody clone 2.4G2 (BioXCell), and 100 μg/mL of polyclonal rat, mouse, and Armenian Hamster IgG (Innovative Research Inc.) in FACS buffer and stained with the antibodies described in Supplementary Table for 30 min in the dark at 4 °C. ..

    Incubation:

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism
    Article Snippet: .. Cells were stained with Zombie-aqua (BioLegend) to discriminate dead cells, incubated with anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, and labeled using fluorescence-conjugated anti-mouse antibodies: CD3ε -PE/Dazzle 594 (clone 145-2C11, dilution 1:200, Cat 100347), CD4-Pacific blue (clone RM4-4, dilution 1:200, Cat 116008), B220-AlexaFluor700 (clone RA3-6B2, dilution 1:200, Cat 103232), CD138-PE (clone 281-2, dilution 1:200 Cat 142504), and CD8-APC/FireTM 750 (clone 53-6.7, dilution 1:200 Cat 100766). .. Data were acquired using a flow cytometer (LSR Fortessa; BD Biosciences, San Jose, CA, USA).

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism
    Article Snippet: .. After incubation with Zombie-Aqua (BioLegend) for dead cell staining and anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, the cells were incubated with 20 μL recipient plasma for 30 min. After washing, the splenocytes were incubated with Alexa Fluor 488-conjugated anti-mouse IgG (A-21202, Thermo Fisher, 1:150) or Alexa Fluor 488-conjugated anti-mouse IgM (ab150121, Abcam, 1:150) immunofluorescent antibody for another 30 min. Data were acquired using a flow cytometer (CytoFlex; Beckman Coulter, Inc.). ..

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism.
    Article Snippet: .. Cells were stained with Zombie-aqua (BioLegend) to discriminate dead cells, incubated with anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, and labeled using fluorescence-conjugated anti-mouse antibodies: CD3ε -PE/Dazzle 594 (clone 145-2C11, dilution 1:200, Cat 100347), CD4-Pacific blue (clone RM4-4, dilution 1:200, Cat 116008), B220AlexaFluor700 (clone RA3-6B2, dilution 1:200, Cat 103232), CD138-PE (clone 281-2, dilution 1:200 Cat 142504), and CD8-APC/FireTM 750 (clone 53-6.7, dilution 1:200 Cat 100766). .. Data were acquired using a flow cytometer (LSR Fortessa; BD Biosciences, San Jose, CA, USA).

    Blocking Assay:

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism
    Article Snippet: .. Cells were stained with Zombie-aqua (BioLegend) to discriminate dead cells, incubated with anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, and labeled using fluorescence-conjugated anti-mouse antibodies: CD3ε -PE/Dazzle 594 (clone 145-2C11, dilution 1:200, Cat 100347), CD4-Pacific blue (clone RM4-4, dilution 1:200, Cat 116008), B220-AlexaFluor700 (clone RA3-6B2, dilution 1:200, Cat 103232), CD138-PE (clone 281-2, dilution 1:200 Cat 142504), and CD8-APC/FireTM 750 (clone 53-6.7, dilution 1:200 Cat 100766). .. Data were acquired using a flow cytometer (LSR Fortessa; BD Biosciences, San Jose, CA, USA).

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism
    Article Snippet: .. After incubation with Zombie-Aqua (BioLegend) for dead cell staining and anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, the cells were incubated with 20 μL recipient plasma for 30 min. After washing, the splenocytes were incubated with Alexa Fluor 488-conjugated anti-mouse IgG (A-21202, Thermo Fisher, 1:150) or Alexa Fluor 488-conjugated anti-mouse IgM (ab150121, Abcam, 1:150) immunofluorescent antibody for another 30 min. Data were acquired using a flow cytometer (CytoFlex; Beckman Coulter, Inc.). ..

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism.
    Article Snippet: .. Cells were stained with Zombie-aqua (BioLegend) to discriminate dead cells, incubated with anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, and labeled using fluorescence-conjugated anti-mouse antibodies: CD3ε -PE/Dazzle 594 (clone 145-2C11, dilution 1:200, Cat 100347), CD4-Pacific blue (clone RM4-4, dilution 1:200, Cat 116008), B220AlexaFluor700 (clone RA3-6B2, dilution 1:200, Cat 103232), CD138-PE (clone 281-2, dilution 1:200 Cat 142504), and CD8-APC/FireTM 750 (clone 53-6.7, dilution 1:200 Cat 100766). .. Data were acquired using a flow cytometer (LSR Fortessa; BD Biosciences, San Jose, CA, USA).

    Labeling:

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism
    Article Snippet: .. Cells were stained with Zombie-aqua (BioLegend) to discriminate dead cells, incubated with anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, and labeled using fluorescence-conjugated anti-mouse antibodies: CD3ε -PE/Dazzle 594 (clone 145-2C11, dilution 1:200, Cat 100347), CD4-Pacific blue (clone RM4-4, dilution 1:200, Cat 116008), B220-AlexaFluor700 (clone RA3-6B2, dilution 1:200, Cat 103232), CD138-PE (clone 281-2, dilution 1:200 Cat 142504), and CD8-APC/FireTM 750 (clone 53-6.7, dilution 1:200 Cat 100766). .. Data were acquired using a flow cytometer (LSR Fortessa; BD Biosciences, San Jose, CA, USA).

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism.
    Article Snippet: .. Cells were stained with Zombie-aqua (BioLegend) to discriminate dead cells, incubated with anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, and labeled using fluorescence-conjugated anti-mouse antibodies: CD3ε -PE/Dazzle 594 (clone 145-2C11, dilution 1:200, Cat 100347), CD4-Pacific blue (clone RM4-4, dilution 1:200, Cat 116008), B220AlexaFluor700 (clone RA3-6B2, dilution 1:200, Cat 103232), CD138-PE (clone 281-2, dilution 1:200 Cat 142504), and CD8-APC/FireTM 750 (clone 53-6.7, dilution 1:200 Cat 100766). .. Data were acquired using a flow cytometer (LSR Fortessa; BD Biosciences, San Jose, CA, USA).

    Fluorescence:

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism
    Article Snippet: .. Cells were stained with Zombie-aqua (BioLegend) to discriminate dead cells, incubated with anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, and labeled using fluorescence-conjugated anti-mouse antibodies: CD3ε -PE/Dazzle 594 (clone 145-2C11, dilution 1:200, Cat 100347), CD4-Pacific blue (clone RM4-4, dilution 1:200, Cat 116008), B220-AlexaFluor700 (clone RA3-6B2, dilution 1:200, Cat 103232), CD138-PE (clone 281-2, dilution 1:200 Cat 142504), and CD8-APC/FireTM 750 (clone 53-6.7, dilution 1:200 Cat 100766). .. Data were acquired using a flow cytometer (LSR Fortessa; BD Biosciences, San Jose, CA, USA).

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism.
    Article Snippet: .. Cells were stained with Zombie-aqua (BioLegend) to discriminate dead cells, incubated with anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, and labeled using fluorescence-conjugated anti-mouse antibodies: CD3ε -PE/Dazzle 594 (clone 145-2C11, dilution 1:200, Cat 100347), CD4-Pacific blue (clone RM4-4, dilution 1:200, Cat 116008), B220AlexaFluor700 (clone RA3-6B2, dilution 1:200, Cat 103232), CD138-PE (clone 281-2, dilution 1:200 Cat 142504), and CD8-APC/FireTM 750 (clone 53-6.7, dilution 1:200 Cat 100766). .. Data were acquired using a flow cytometer (LSR Fortessa; BD Biosciences, San Jose, CA, USA).

    Cell Culture:

    Article Title: Myeloid-Derived Suppressor Cells Induce Exhaustion-Like CD8 + T Cells during JEV Infection
    Article Snippet: .. Single-cell suspensions from the mouse spleen, lymph node, blood, and cultured cells were preincubated for 10 min at room temperature with 0.3 μg of anti-CD16/CD32 antibody (BioXcell). ..

    Clinical Proteomics:

    Article Title: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism
    Article Snippet: .. After incubation with Zombie-Aqua (BioLegend) for dead cell staining and anti-CD16/CD32 antibody (Bio X Cell) to block Fc receptors, the cells were incubated with 20 μL recipient plasma for 30 min. After washing, the splenocytes were incubated with Alexa Fluor 488-conjugated anti-mouse IgG (A-21202, Thermo Fisher, 1:150) or Alexa Fluor 488-conjugated anti-mouse IgM (ab150121, Abcam, 1:150) immunofluorescent antibody for another 30 min. Data were acquired using a flow cytometer (CytoFlex; Beckman Coulter, Inc.). ..

    FACS:

    Article Title: PD-L1- and IL-4-expressing basophils promote pathogenic accumulation of T follicular helper cells in lupus
    Article Snippet: For mouse samples, cells washed in PBS were stained with GHOST 510 viability dye (TONBO) following the manufacturer’s instructions. .. Non-specific antibody binding sites were saturated with 10 μg/mL of anti-CD16/CD32 antibody clone 2.4G2 (BioXCell), and 100 μg/mL of polyclonal rat, mouse, and Armenian Hamster IgG (Innovative Research Inc.) in FACS buffer and stained with the antibodies described in Supplementary Table for 30 min in the dark at 4 °C. ..



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